Determination of Beneficial Effects of Salbutamol and Ipratropium in Prevention of Insulin Induced Tracheal Smooth Muscle Contraction in Guinea Pig Model: A Comparative Study

Aim use of inhalational insulin was limited due to its high cost and its potential to produce respiratory adverse effects. So current study was designed to evaluate the acute effects of insulin on airway reactivity and protective effects of salbutamol and ipratropium against insulin induced airway hyper-responsiveness on isolated tracheal smooth muscle of guinea pig. Material and methods: effects of variable doses of insulin (10 to 10 M), insulin pretreated with fixed concentration of salbutamol (10 M) and ipratropium (10 M) 7 3 7 6 were studied on isolated tracheal tissue of guinea pig by constructing cumulative concentration response curves. Changes in tracheal smooth muscle contractions were recorded on four channel oscillograph. Results, the maximum amplitude of contraction with increasing concentrations of insulin, insulin pretreated with fixed concentration of salbutamol and ipratropium were 35±1.13mm, 14.55±0.62mm and 27.8±1.27mm respectively. Salbutamol inhibited the contractile response of insulin greater than ipratropium on isolated tracheal muscle of guinea pig. Conclusion maximum constrictor response of insulin in the presence of salbutamol and ipratropium was reduced by 41.57 and 79.42 percent respectively as compared with control group so we suggest that pretreatment of inhaled insulin with salbutamol may be preferred over ipratropium in amelioration of its potential respiratory adverse effects.